Dr. Jiao Zhai

Dr. Jiao Zhai |Clyto Access

Chinese Academy of Science, China


Expertise: Nanocluster, Cytometry, Biomarkers, Nanosafety, Nanohyrid

Biography: Dr. Jiao Zhai, MD-PhD-student (2012-2017) in biological inorganic chemistry. Now is a PhD student of Key Laboratory for Biomedical Effects of Nanomaterials and Nanosafety, Chinese Academy of Science. Currently Dr. Jiao Zhai’ researches focus on developing peptide mimic method to synthesize novel inorganic-biomolecule nanohybrids to detect the cancer biomarkers in living cell in situ. And Dr. Jiao Zhai got the outstanding student Award in 2015 and 2016,


Title: Construction of Au nanocluster probe for single cell protein detection


The research of protein on a single-cell level would aid the studies of the role of cellular heterogeneity in disease progression. A few methods, such as flow cytometry, mass cytometry, have realized single-cell protein quantification. However, the accuracy of the quantification is limited. In this study, we designed an Au nanocluster probe with the formula of Au24Peptide8, which has both fluorescence property and specific targeting ability of integrin αIIbβ3. Utilizing the fluorescence property and targeting ability, we can directly visualize αIIbβ3 on the membrane of human erythroleukemia cells (HEL) via confocal microscopy. On the basis of the accurate formula of our probe (Au24Peptide8), the number of integrin αIIbβ3 can be precisely counted by quantifying the gold content on a single HEL cell via laser ablation inductively coupled plasma mass spectrometry. The expression amount of integrin αIIbβ3 on single HEL cells range from 5.75 × 10−17 to 9.11 × 10−17 mol. Because the different expression level of protein (e.g. biomarker) on single cells is related to the occurrence and development of disease, our method will contribute to estimate the progression of disease by precisely quantifying any well-identified protein related to the disease. This method will offer new perspectives in disease diagnosis and therapy on a single-cell level.


Related Conferences :

World Summit on Nanotechnology and Nanomedicine Research