Dr. Reyhane Hoshyar

Dr. Reyhane Hoshyar |Clyto Access

Birjand University, Iran

Organizing Committee Member

Expertise: Cell Signaling, Gene Expression, Cancer Stem, Cell Biology


Dr. Reyhane Hoshyar carried out her post -doctoral research at Tarbiat Modares University of Tehran, Iran. Later she worked as a scholar visitor at Massachusetts University in Amherst, USA.
In addition to experience with most molecular biological techniques, her dissertation work involved the study of apoptotic effects of some herbal components on several cancers, in 
vitro and in vivo. Presently she working as an assistant professor in Birjand University of Medical Sciences, Iran.


Title: Anticancer and Apoptotic Effects of Crocetin on Hepatocellular Carcinoma HepG2 Cells


Introduction: Hepatocellular carcinoma is the sixth most common cancer, and the third most common cause of cancer-related death worldwide. Saffron is the red dried stigma of Crocus sativus L. herb which has various medicinal properties such as anticancer activity. The molecular mechanism of its active metabolites, for instance crocetin that causes cytotoxicity in tumor cells, have not been investigated in detail. In the present study, the effects of crocetin on proliferation and apoptosis of HepG2 cells, a human liver cáncer cell line, were evaluated.

Methods: The cells were treated with different concentrations of crocetin (0-1 mg/mL) for various times (0-72 h). Cell viability and apoptosis were assessed by MTT assay, Hoechst 33258 staining, and caspase activity, respectively. The mRNA levels Bax, and Bcl-2 were measured using quantitative real-time polymerase chain reaction (qRT- PCR). Results: Our data showed that crocetin inhibited the growth of HepG2 cells in a dose- and time-dependent manner. Altered nuclear morphology and increased caspase activity (6-fold) indicated that crocetin notably induced apoptosis in these cells. Results of Hoechst staining also indicated that crocetin increased apoptosis in a dose-dependent manner (41% and 65% of apoptotic cells of total cells after 0.2 and 0.5 mg/mL crocetin treatments, respectively). Furthermore, there was a marked increase (3-fold) in the Bax/Bcl-2 ratio for mRNA levels, an apoptotic index,) in these cells after crocetin treatment.

Conclusion: Crocetin effectively suppressed the proliferation of HepG2 cells and induced apoptosis. This study demonstrates strong antiproliferative and apoptotic activities of crocetin against liver cancer cells. Hence, it is suggested that more studies are warranted to evaluate the pharmacological activities of crocetin as a possible, safe, and promising anticancer agent in cancer.

Related Conferences :

International Conference on Cancer Care and Cure