Mr. Takahiro Abe

Mr. Takahiro Abe |Clyto Access

Visiting Researcher, Kanagawa University, Japan

Poster Presentation

Expertise: Marine Biotechnology and Microbiology

Biography:

Mr. Takahiro abe is cuurently working as a visiting researcher at the Department of Research Institute of Natural Drug-Leads in Kanagawa University, Japan. He has published 4 research articles in various reputed national and international journals.

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Presentation:

Title: Isolation of halichrome A from a Metagenomic Library Derived from the Marine Sponge Halichondria okadai

Abstract:

In the ocean, many structurally unique compounds that have significant biological activities have been isolated from various marine invertebrates. Especially, sponges, belonging to the porifera, are also isolated many natural products. Recent research suggests that many marine sponges harbor various microbial symbionts, by which many bioactive compounds are produced, and that the number of cultivable bacteria represents 1% or fewer of the total environmental bacteria. So, to take advantage of sponge symbionts efficiently, the metagenomic analysis is optimal as culture independent analysis technique. In this study, we isolate metagenomic DNA from bacterial symbionts of Japanese marine sponge, H. okadai, and isolated a pigment from a metagenomic library. Marine sponge, H. okadai, was crushed with buffer and separated by centrifuge and the genomic DNA was extracted from bacteria fraction. Firstly, metagenomic DNA was ligated into fosmid vector pCC1FOS vector (Epicentre) and the ligated vectors were transformed into E. coli EPI300 (Epicentre). The transformants were spread onto LB medium. A total of 150000 fosmid clones metagenomic library were constructed. Then, isolated genomic DNA was ligated into BAC vector pCC1BAC (Epicentre) and the ligated vectors were transformed into E. coli EPI100. Constructed fosmid library derived from H. okadai, was screened for pigment production to obtain several active clones. Then, tinted clones were screened and the compound was purified from culture broth of clone by several chromatography steps to afford single isolated compound. We clarified that yellow pigment shows moderate cytotoxicity to B16 cell. We isolated the novel pigment halichrome A, which was determined to be a biindole, and which exhibited cytotoxicity against B16 melanoma cells.

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Related Conferences :

Marine Science Research and Technology Conference