Ms.Sandhya Chipurupalli

Ms.Sandhya Chipurupalli |Clyto Access

Research Scholar, JSS College of Pharmacy, India.

Poster Presentation

Expertise: Cancer cells, Diabetes, Cell biology

Biography:

Ms. Sandhya Chipurupalli Master of Pharmacy (M. Ph) in Pharmacology from Sri Sai Aditya Inst. Of Pharmaceutical Sciences & Research,  Affiliated to Andhra University, India. Later she started working as a assistant professor in VJ’S college of Pharmacy (Andhra University), Rajahmundry, E.G.Dt, A.P, India. Presently she is working as Research Scholar, Department of Pharmacology, JSS College of Pharmacy, India.

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Presentation:

Title: Effect of Triterpene Fraction ofCucumistrigonus on Apoptosis, Cell Cycle Arrest and Cell Proliferation in Cancer Cells

Abstract:

Abstract:

Taxol based combination chemotherapy remains the predominant treatment for cancer. However, taxane related drug resistance and neurotoxicity have promoted us to develop substitute treatment strategies. Kinesis spindle protein which is crucial for bipolar spindle formation and duplicate chromosome separation in early phase of mitosis, emerged as  attractive target for cancer chemotherapy. The aim of the study was to investigate the mechanism of kinesis spindle protein inhibition that plays an essential role in establishing mitotic spindle bipolarity, by the novel small molecular inhibitor.Antiproliferative activity of triterpene fraction ofCucumistrigonus(TF25CT) in cancer cell line was accessed by cell viability assay(MTT, SRB). TF25CT showed dose dependent inhibition of cancer cell proliferation. The antiproliferative effect of TF25CT was associated with induction of apoptosis as well as morphology changes and DNA fragment. The morphology of Apoptosis nuclei was quantified using Hoechst staining and PI fragmentation. The degree of DNA fragmentation was analyzed by agarose gel electrophoresis. The observations indicate that induced apoptotic- like morphological changes such as cell shrinkage and blebbing. Suppression of microtubule dynamics is responsible for the ability of TF25CT to inhibit the mitotic progression and cell proliferation using fluorescent microscope, we examined the effect of TF25CT on dynamics of individual microtubule in two living human cancer cell line. Collectively, the kinesis protein inhibitor represents an importedcompound for further investigation as novel anticancer remedial.

Key words:Antiproliferation, Apoptosis, Cucumistrigonus, Cancer cells, Kinesis protein
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Related Conferences :

International Conference on Cancer Care and Cure