Ms.Selivanova Elena

Ms.Selivanova Elena |Clyto Access

National Medical Research Radiological Centre of the Ministry of Health of the Russian Federation

Poster Presentation

Expertise: Somatic mutations, radioadaptive and bystander effects, the effect of small doses of radiation on cellular immunity, tumor resistance to radiotherary

Biography:

Ms. Elena I. Selivanova was carried her  diploma in Moscow State University of M. V. Lomonosov and Ph. D in Radiation Biology. Title of the PhD thesis:  Determination of somatic mutant frequencies of  Glycophorin A loci of person   exposed to ionizing radiation”. Later she started working as a teacher  and research assistant in the Department of Biochemistry of Medical Radiological Research Center of the Russian Academy of Medical Sciences and presently she is working as a senior scientist researcher in the Department of Biochemistry of A.Tsyb Medical Radiological Research Center, branch of the National Medical Research Radiological Center , Ministry of Health of the Russian Federation, Obninsk, Russia.

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Presentation:

Title: Conjugates of PAMAM dendrimers with doxorubicin and vector protein decrease survival of cancer cells demonstrating resistance to traditional anticancer agents in vitro

Abstract: Development of new systems for  selective transport оf anticancer agents in tumor cells on the base of dendritic polymers (dendrimers) is appeared to be one of the most effective approaches to  overcome cancer chemo resistance associated with the existence of   cancer stem cells  (CSCs)   and multidrug  resistance (MDR).  The aim of this work was to study cytotoxic effects  of polyamideamine  dendrimers of the second generation (G2) covalently conjugated to doxorubicin (Dox) and a vector protein (the third recombinant domain of the alpha-fetoprotein - 3D AFP) on two breast cancer cell lines (MCF-7 and MCF-7/MDR1) differing in sensitivity to anticancer drugs and to compare sensitivity of  CSCs and non-CSCs to these conjugates. The cytotoxic effects were assessed after incubation with free Dox,  G2-Dox and  3D-G2-Dox at concentration of 2.5 µm for Dox using clonogenic test. CSCs were identified in MCF-7 line by flow cytometry as side population of Hoescht33342-excluding cells 24 hours after incubation  with these agents.  3D-G2-Dox significantly decreased the clonogenity of MCF-7/MDR1 cells (19% in comparison to control, p<0.05)  in contrast to the MCF-7 cells (70%).  Free Dox was effective for elimination only MCF-7 cells (1%, p<0.001), but not MCF-7/MDR cells (78%). In addition, 3D-G2-Dox was able to decrease the proportion of MCF-7 CSCs in contrast to free Dox that greatly increased the proportion of such cells. The results show promising use of dendrimers as nano-carriers of  anticancer drugs for overcome tumor cell chemo resistance.,

Related Conferences :

International Conference on Cancer Care and Cure